Structure of a Plant Cell Wall Fragment Complexed to Pectate Lyase C
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چکیده
منابع مشابه
Structure of a plant cell wall fragment complexed to pectate lyase C.
The three-dimensional structure of a complex between the pectate lyase C (PelC) R218K mutant and a plant cell wall fragment has been determined by x-ray diffraction techniques to a resolution of 2.2 A and refined to a crystallographic R factor of 18.6%. The oligosaccharide substrate, alpha-D-GalpA-([1-->4]-alpha-D-GalpA)3-(1-->4)-D-GalpA , is composed of five galacturonopyranose units (D-GalpA)...
متن کاملStructure of pectate lyase A: comparison to other isoforms.
Pectate lyase A is a virulence factor secreted by the plant-pathogenic bacteria Erwinia chrysanthemi. The enzyme cleaves the glycosidic bond of pectate polymers by a calcium-dependent beta-elimination mechanism. The crystal structure of pectate lyase A from E. chrysanthemi EC16 has been determined in two crystal forms, monoclinic C2 to 1.8 A and rhombohedral R3 to 2.1 A. The protein structure i...
متن کاملThree - Dimensional Structure of Pectate lyase E , a Plant Virulence Factor from Erwinia chrysanthemi '
The three-dimensional structure of pectate lyase E (PelE) has bee? determined by crystallographic techniques at a resolution of 2.2 A. The model includes all 355 amino acids but no solvent, and refines to a crystallographic refinement factor of 20.6%. The polypeptide backbone folds into a large right-handed cylinder, termed a parallel @ helix. loops of various sizes and conformations protrude f...
متن کاملThe Three-Dimensional Structure of Pectate Lyase E, a Plant Virulence Factor from Erwinia chrysanthemi.
The three-dimensional structure of pectate lyase E (PelE) has been determined by crystallographic techniques at a resolution of 2.2 A. The model includes all 355 amino acids but no solvent, and refines to a crystallographic refinement factor of 20.6%. The polypeptide backbone folds into a large right-handed cylinder, termed a parallel [beta] helix. Loops of various sizes and conformations protr...
متن کاملThe Refined Three-Dimensional Structure of Pectate Lyase
The crystal structure of pectate lyase E (PelE; EC 4.2.2.2) from the enterobacteria Erwinia cbrysanfbemi has been refined by molecular dynamics techniques to a resolution of 2.2 a and an R factor (an agreement factor between observed structure factor amplitudes) of 16.1%. The final model consists of all 355 amino acids and 157 water yolecules. The root-mean-square deviation from ideality is 0.0...
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ژورنال
عنوان ژورنال: The Plant Cell
سال: 1999
ISSN: 1040-4651,1532-298X
DOI: 10.1105/tpc.11.6.1081